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Stress can manifest as a physical barrier, ultraviolet light, or a toxic substance such as antibiotics. The formation of these biofilms takes from several hours to days, and they can be of different shapes, sizes, colors, and textures depending on the type of bacteria.
Being in a biofilm state protects them from harmful substances in the environment – biofilms have a unique outer wall with different physical and chemical properties than their individual cells. They can coordinate metabolism, slow down their growth, and even form an impenetrable barrier of wrinkles and creases.
This is one of the ways they achieve high antibiotic resistance. Researchers from the United Kingdom recently studied the transition of the Hay Bacillus bacterium from a free-moving swarm to a biofilm as a defense mechanism and published what they did to combat its antibiotic-resistant properties in eLife.
To determine if their test strain was behaving like the others, they first ran stress tests on them. They tested the bacteria’s response to the physical barrier, ultraviolet light, and antibiotics. The addition of a physical barrier led to the transition of bacteria from one layer to a multilayer layer, followed by an increase in cell density and the formation of multilayer islands near the barrier.
Later, wrinkles formed on the islands near the barrier in the place where they began to appear initially. And after the scientists added a large dose of the antibiotic kanamycin, the bacterial cells formed a biofilm.
The researchers then developed a strategy to combat this bacterial biofilm. They added kanamycin to the environment of a new batch of swarming bacterial cells and watched as the biofilm began to form.
They then re-injected the antibiotic at a much higher dose than the first, just before the biofilm formation was complete. As a result, the partially formed biofilm was destroyed and bacterial cells died.
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